Environmental DNA (eDNA) Coordination Statement of Interest is sponsored by New York State Department of State. Seeks proposals for coordinating environmental DNA studies, relevant to environmental and climate research.

NYC/NJ Aquatic Vertebrate Environmental DNA (eDNA) Project – The Rockefeller University – Program for the Human Environment Program for the Human Environment NYC/NJ Aquatic Environmental DNA (eDNA) Project We aim to help advance eDNA for monitoring ocean life. Our research focuses on eDNA assessment of presence and abundance of marine fish in New York and New Jersey.

We have also applied eDNA to freshwater fish, marine mammals, and terrestrial vertebrates, at diverse sites ranging from Sea of Galilee in Israel to twilight zone ocean at edge of eastern US continental shelf. Why eDNA? Ocean life is largely hidden from view.

Traditional surveys for marine animals are costly, time- and capital-intensive, needing special equipment and trained personnel. Surveys are relatively sparse, and many regions remain unexplored. eDNA offers a low-cost, non-destructive complement to traditional surveys, with significant added value in otherwise difficult-to-survey environments, for protected species, and if pandemics or other events curtail routine surveys.

eDNA will help monitor impacts of human activities, weather and climate, conservation efforts, and will aid research and exploration. Optimizing eDNA methods for metabarcoding marine fish. eDNA metabarcoding uses PCR primers that amplify the DNA of multiple species in a taxonomic group, e.g., vertebrates.

The amplified DNA is analyzed by high-throughput NextGen sequencing, and the resulting “reads” are sorted and matched to a reference library of sequences from identified fish specimens. We have shown that current laboratory protocols optimize sensitivity and reproducibility, especially for more abundant fish populations ( ICES J Mar Sci 2022a ). We conclude that eDNA rarity poses the main challenge to current methods.

eDNA metabarcoding has generally been considered best suited for presence/absence assessment rather than as a measure of abundance. We recently demonstrated that with certain primer sets, spiking metabarcoding PCRs with an exogenous standard (e.g., ostrich DNA as illustrated below) enables accurate measurement of eDNA copy number levels ( ICES J Mar Sci 2022b ).

This opens the door to comparing fish abundance among species, by habitat and season, and monitoring response to restoration efforts. Our work in this area is currently supported by NOAA Award Number NA23OAR0110593.

_______________________________________________________________________________________ Marine eDNA 101 bookl et pdf (618 KB) _______________________________________________________________________________________ In collaboration with Monmouth University and New Jersey Department of Environmental Protection, we conducted one of the largest comparisons of eDNA and bottom trawling to date.

We found the two technologies were largely concordant on marine fish diversity, seasonality, and relative abundance (link to article ICES J Marine Sci 2021 , also see Science online coverage by Erik Stokstad). ______________________________________________________________________________________ Together with Monmouth University, we hosted the first National Conference on Marine Environmental DNA , November 29-30, 2018.

The Conference included approximately 100 American ocean scientists and associated stakeholders, including representatives from academe, federal, state, and local governments, non-governmental organizations concerned with marine environment, and the private sector. The strong sense of the meeting was “eDNA works–let’s get going.

” The Conference Final Report and press release summarize the conference proceedings and outline concrete steps forward. The Conference attracted coverage in Science online and print , and National Geographic online , reflecting the excitement around marine eDNA for monitoring and exploring ocean life.

_______________________________________________________________________________________ Bay fish eDNA in bay, ocean fish eDNA in ocean Contact: Mark Stoeckle mark. stoeckle@rockefeller. edu Site updated November 2020 More aquatic eDNA projects 2015 NYC Marine eDNA survey 2015 eDNA NYC Central Park Mark Y.

Stoeckle, Jesse H. Ausubel, Michael Coogan. 12S gene metabarcoding with DNA standard quantifies marine bony fish environmental DNA, identifies threshold for reproducible detection, and overcomes distortion due to amplification of non-fish DNA (PDF).

, Environmental DNA, (eds.) DOI: 10. 1002/edn3.

376 (11 November): 2022 Stoeckle MY, Adolf J, Ausubel JH, Charlop-Powers Z, Dunton K, Hinks G. Current laboratory protocols for detecting fish species with environmental DNA optimize sensitivity and reproducibility, especially for more abundant populations [open access external link]. ICES J Marine Sci, 2022.

Stoeckle MY, Adolf J, Charlop-Powers Z, Dunton K, Hinks G, VanMorter SM. Trawl and eDNA assessment of marine fish in coastal New Jersey, USA [open access external link]. ICES J Marine Sci, 2021.

Stoeckle MY, Mishu MD, Charlop-Powers Z. Improved Environmental DNA Reference Library Detects Overlooked Marine Fishes in New Jersey, United States [open access external link]. Frontiers in Marine Science 7 (226): 2020 Stoeckle MY, Mishu M, Charlop-Powers Z.

GoFish: a versatile strategy for nested PCR environmental DNA assays for marine vertebrates [open access external link]. PLOS ONE 2018:e0198717. Stoeckle MY.

Fishing for DNA: free-floating eDNA identifies presence and abundance of ocean life [open access external link]. The Conversation, April 12, 2017. Stoeckle MY, Soboleva L, Charlop-Powers Z.

Aquatic environmental DNA detects seasonal fish abundance and habitat preference in an urban estuary [open access external link]. PLOS ONE 2017: e0175186. About the Bar Code of Life site This web site is an outgrowth of the Taxonomy, DNA, and Barcode of Life meeting held at Banbury Center, Cold Spring Harbor Laboratory, September 9-12, 2003.

It is managed by Mark Stoeckle at the Program for the Human Environment (PHE) at The Rockefeller University. Contact: mark. stoeckle@rockefeller.

edu for the Human Environment The involvement of the Program for the Human Environment in DNA barcoding dates to Jesse Ausubel's attendance in February 2002 at a conference in Nova Scotia organized by the Canadian Center for Marine Biodiversity. At the conference, Paul Hebert presented for the first time his concept of large-scale DNA barcoding for species identification.

Impressed by the potential for this technology to address difficult challenges in the Census of Marine Life, Jesse agreed with Paul on encouraging a conference to explore the contribution taxonomy and DNA could make to the Census as well as other large-scale terrestrial efforts.

In his capacity as a Program Director of the Sloan Foundation, Jesse turned to the Banbury Conference Center of Cold Spring Harbor Laboratory, whose leader Jan Witkowski prepared a strong proposal to explore both the scientific reliability of barcoding and the processes that might bring it to broad application. Concurrently, PHE researcher Mark Stoeckle began to work with the Hebert lab on analytic studies of barcoding in birds.

Our involvement in barcoding now takes 3 forms: assisting the organizational development of the Consortium for the Barcode of Life and the Barcode of Life Initiative; contributing to the scientific development of the field, especially by studies in birds, and contributing to public understanding of the science and technology of barcoding and its applications through improved visualization techniques and preparation of brochures and other broadly accessible means, including this website.

While the Sloan Foundation continues to support CBOL through a grant to the Smithsonian Institution, it does not provide financial support for barcoding research itself or support to the PHE for its research in this field. What's New in DNA Barcoding