CASCADE Initiative

Biotech routes for valorisation of residual biomass is sponsored by European Commission — Horizon Europe. Expected Outcome: Successful proposals will contribute to the implementation of the EU initiative on Biotechnology and Biomanufacturing and the EU Life Sciences Strategy as well as the updated EU Bioeconomy Strategy, the Clean Industrial Deal, the European Chemical Industry Action Plan, the upcoming EU Biotechnology and Circular Economy Acts. Projects results are expected to contribute to the following expected outcomes: Increased added value of residual biomass for biorefinery applications. Availability of a wider portfolio of sustainable bio-based products via industrial biotech. Robust, scalable and efficient biotech process(es) applicable to residual biomass. Scope: Diversifying biomass feedstock resources for bio-based products is key to contribute to resilience and strategic autonomy of bio-based operations. Industrial biotechnologies have showcased that they can play a key role to address residual biomass into valuable bio-based products. However, scaling up from research to demo scale requires addressing systemic and technical challenges. This entails the integration of adequate pretreatment and conversion processes, tailored to the targeted residual biomass and its composition variability, in order to achieve high yield, titre and selectivity. At the same time, it requires that these processes are scalable and sustainable. Moreover, effective logistics are needed to mobilise the residual biomass, requiring cooperation with primary producers, waste management operators and other biomass providers. Proposals under this topic should: Demonstrate (at least TRL 6) efficient biotechnology based processes to convert residual biomass streams into bio-based chemicals, intermediates, polymers, materials, ingredients [1] and/or enzymes. The topic focuses on processes in which biotechnology is the key enabling technology; the integration of supporting unit operations based on technologies other than biotechnology is in scope. Optimisation of selected cells, enzymes and/or microorganisms, including microbiomes, is also in scope. The feedstock in scope includes: Forestry and agricultural residues and/or side streams from the processing of forestry and agricultural biomass (including livestock-based ones such as manure and animal byproducts), residues from aquatic biomass, including from fisheries, micro/macro algae production and aquaculture, urban and/or industrial bio-based waste and side-streams (including food industry waste and side-streams, cellulose from post-consumer and post-industrial waste, urban biowaste and sewage sludge, and other bio-based waste/side-streams from industrial operations including wood and pulp and paper industry), mixed streams from the above. Integrate optimised biomass pretreatment/fractionation processes tailored to selected residual biomass and optimise (energy)-efficient separation and/or purification process step(s) across the value chain. The valorisation of by-products and side streams across the value chain via the cascading approach is in scope. Validate (at TRL 5 and above) conversion (or use) of biorefinery product(s) into end-products proving to fulfil market requirements for selected applications sectors. Address resource efficiency and circularity. When pursuing circular models, ensure that neither pathogens nor contaminants are injected back in the loop, to avoid negative effects on human health and the environment. In addition to the specific requirements applicable for the type of action, as described in section 2.2.3.1 of the CBE JU Annual Work Programme 2026 [2] , proposals under this topic should: Under the multi-actor approach (MAA), ensure the sufficient involvement of feedstock producers/managers for residual feedstock supply. Optimise logistic models (including sourcing, storage and transport) of residual feedstock, minimising distances and costs. Ensure seamless integration with biomass providers, while promoting win-win business models. Perform a risk assessment on impacts, p Programme areas: Global Challenges and European Industrial Competitiveness, Food, Bioeconomy Natural Resources, Agriculture and Environment, Bio-based Innovation Systems in the EU Bioeconomy, Horizon Europe (HORIZON) Keywords: Applied and industrial chemistry, Bioprocessing technologies (industrial processes relying on biological agents to drive the process) biocatalysis, fermentation, Industrial bioengineering, Industrial biotechnology, bio-based chemicals, bio-based ingredients, bio-based materials, bio-based polymers, bio-based products, bio-conversions, biotechnology, STEP-Biotech

Diversification of nutritional food ingredient sources for increased EU resilience and strategic autonomy is sponsored by European Commission — Horizon Europe. Expected Outcome: Successful proposals will contribute to the implementation of the EU initiative on Biotechnology and Biomanufacturing and the EU Life Sciences Strategy as well as the updated EU Bioeconomy Strategy, the upcoming EU Biotechnology Act and EU Circular Economy Act. Projects’ results are expected to contribute to the following expected outcomes: Full industrial scale biorefinery and related value chain(s) for the production of nutritional food ingredients. Resilience and strategic autonomy of EU food sectors via diversification of nutritional food ingredient sources. Increased environmental sustainability of food sectors (e.g. addressing issues like land use, water use, energy consumption, nitrogen cycle, other nutrients, etc.). Improved consumers awareness and acceptance of nutritional food ingredients from alternative sources, contributing to sustainable healthy diets. Scope: Human nutrition is a key area where the bio-based industries can play an important role in addressing the present societal and climate challenges. Considerable attention is given to the utilisation of alternative sources of proteins, fibres and oils/fats, due to the increasing world population and the pressure on finite natural resources. Many sources for food ingredients, alternative to agricultural crops, exist and have been successfully piloted across the EU. However, efforts are needed to scale up sustainable processes to achieve adequate or even improved nutritional properties, ensure safety and consumers/end users acceptance, while achieving cost competitiveness. Proposals under this topic should: Demonstrate (at TRL 8) the efficient production of nutritional ingredients for food applications. Proteins, lipids, specialty carbohydrates, and fibres are in scope. Target at least one of these as the main product driving the business case. Structural or functional ingredients such as colourants, preservatives, stabilisers, texturisers, enzymes are not in scope as the main product. Synergistic co-production of multiple and different food and feed ingredients and other bio-based products is also in scope following the cascading approach. All sources of bio-based feedstock [1] are in scope. Direct production of food from food crops, livestock, fisheries and aquaculture is not in scope. The use of industrial grade feedstock [2] from agricultural crops is in scope for conversion into food grade ingredients. Validate (at TRL 6 and above) the use of the obtained nutritional food ingredient(s) into the formulation of at least 1 food product proving quality, stability, nutritional and sensorial properties. Additional aspects related to prevention of intolerances/allergies, improved palatability and digestibility, health benefits, etc. are also in scope depending on the ingredient(s), formulation(s) and product(s) developed. Address resource efficiency and circularity aspects to increase economic and socio-environmental added value. When pursuing circular models, ensure that neither pathogens nor contaminants are injected back in the loop, to avoid negative toxicological effects. In addition to the specific requirements applicable for the type of action, as described in section 2.2.3.1 of the CBE JU Annual Work Programme 2026 [3] , proposals under this topic should: Assess the safety of developed nutritional food ingredients, in line with EU regulatory requirements and EFSA guidance documents. Moreover, identify potential EU regulatory gaps and propose recommendations to relevant EU policymakers on how to better support food ingredient companies (including startups and scaleups) in addressing EFSA risk assessment. As part of the multi-actor approach (MAA), involve end-users (including consumers) and other relevant actors starting from the early stages to gather input, raise awareness and ultimately foster market acceptance of the targeted end-products and incorporate insights into product development. Ensure complementarities and avoid overlaps with Programme areas: Global Challenges and European Industrial Competitiveness, Food, Bioeconomy Natural Resources, Agriculture and Environment, Bio-based Innovation Systems in the EU Bioeconomy, Horizon Europe (HORIZON) Keywords: Agricultural biotechnology and food biotechnology, Agricultural engineering, food safety, Food biotechnology, alternative sources, carbohydrates, circular economy, fibres, food ingredients, food products, food safety, lipids, nutrition, proteins, STEP-Biotech